Comparative analysis of Phytophthora infestans induced gene expression in potato cultivars with different levels of resistance.
Identifieur interne : 002214 ( Main/Exploration ); précédent : 002213; suivant : 002215Comparative analysis of Phytophthora infestans induced gene expression in potato cultivars with different levels of resistance.
Auteurs : B. Ros [Allemagne] ; F. Thümmler ; G. WenzelSource :
- Plant biology (Stuttgart, Germany) [ 1435-8603 ] ; 2005.
Descripteurs français
- KwdFr :
- Activation de la transcription (MeSH), Analyse de profil d'expression de gènes (MeSH), Maladies des plantes (microbiologie), Phytophthora (physiologie), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (MeSH), Régulation positive (MeSH), Solanum tuberosum (génétique), Solanum tuberosum (microbiologie).
- MESH :
- génétique : Solanum tuberosum.
- microbiologie : Maladies des plantes, Solanum tuberosum.
- physiologie : Phytophthora.
- Activation de la transcription, Analyse de profil d'expression de gènes, Régulation de l'expression des gènes végétaux, Régulation négative, Régulation positive.
English descriptors
- KwdEn :
- MESH :
- genetics : Solanum tuberosum.
- microbiology : Plant Diseases, Solanum tuberosum.
- physiology : Phytophthora.
- Down-Regulation, Gene Expression Profiling, Gene Expression Regulation, Plant, Transcriptional Activation, Up-Regulation.
Abstract
Differential gene expression was analyzed after infection with Phytophthora infestans in six potato cultivars with different levels of resistance to late blight. To verify the infection of the potato leaflets, the amount of phytopathogen mRNA within the plant material was quantified by real-time quantitative PCR. The expression of 182 genes selected from two subtracted cDNA libraries was studied with cDNA array hybridization using RNA from non-infected and infected potato leaflets. Gene up- and down-regulation were clearly detectable in all cultivars 72 h post inoculation. Gene expression patterns in susceptible cultivars differed from those in potato varieties with a higher level of resistance. In general, a stronger gene induction was observed in the susceptible cultivars compared to the moderately to highly resistant potato varieties. Five genes with the highest homology to stress and/or defence-related genes were induced specifically in the susceptible cultivars. Four genes responded to pathogen attack independently of the level of resistance of the cultivar used, and three genes were repressed in infected tissue of most cultivars. Even in the absence of P. infestans infection, six genes showed higher expression levels in the somewhat resistant cultivars Bettina and Matilda. Possible reasons for the different levels of gene expression are discussed.
DOI: 10.1055/s-2005-872946
PubMed: 16388472
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Ros</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Sciences, Center for Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 2, 85350 Freising, Germany. barbara.ros@wzw.tum.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Sciences, Center for Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 2, 85350 Freising</wicri:regionArea><wicri:noRegion>85350 Freising</wicri:noRegion><orgName type="university">Université technique de Munich</orgName><placeName><settlement type="city">Munich</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region></placeName></affiliation></author><author><name sortKey="Thummler, F" sort="Thummler, F" uniqKey="Thummler F" first="F" last="Thümmler">F. Thümmler</name></author><author><name sortKey="Wenzel, G" sort="Wenzel, G" uniqKey="Wenzel G" first="G" last="Wenzel">G. Wenzel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16388472</idno><idno type="pmid">16388472</idno><idno type="doi">10.1055/s-2005-872946</idno><idno type="wicri:Area/Main/Corpus">002184</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002184</idno><idno type="wicri:Area/Main/Curation">002184</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002184</idno><idno type="wicri:Area/Main/Exploration">002184</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative analysis of Phytophthora infestans induced gene expression in potato cultivars with different levels of resistance.</title><author><name sortKey="Ros, B" sort="Ros, B" uniqKey="Ros B" first="B" last="Ros">B. Ros</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Sciences, Center for Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 2, 85350 Freising, Germany. barbara.ros@wzw.tum.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Sciences, Center for Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 2, 85350 Freising</wicri:regionArea><wicri:noRegion>85350 Freising</wicri:noRegion><orgName type="university">Université technique de Munich</orgName><placeName><settlement type="city">Munich</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region></placeName></affiliation></author><author><name sortKey="Thummler, F" sort="Thummler, F" uniqKey="Thummler F" first="F" last="Thümmler">F. Thümmler</name></author><author><name sortKey="Wenzel, G" sort="Wenzel, G" uniqKey="Wenzel G" first="G" last="Wenzel">G. Wenzel</name></author></analytic><series><title level="j">Plant biology (Stuttgart, Germany)</title><idno type="ISSN">1435-8603</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Down-Regulation (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (microbiology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (microbiology)</term><term>Transcriptional Activation (MeSH)</term><term>Up-Regulation (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (physiologie)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (MeSH)</term><term>Régulation positive (MeSH)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (microbiologie)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Transcriptional Activation</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation de la transcription</term><term>Analyse de profil d'expression de gènes</term><term>Régulation de l'expression des gènes végétaux</term><term>Régulation négative</term><term>Régulation positive</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Differential gene expression was analyzed after infection with Phytophthora infestans in six potato cultivars with different levels of resistance to late blight. To verify the infection of the potato leaflets, the amount of phytopathogen mRNA within the plant material was quantified by real-time quantitative PCR. The expression of 182 genes selected from two subtracted cDNA libraries was studied with cDNA array hybridization using RNA from non-infected and infected potato leaflets. Gene up- and down-regulation were clearly detectable in all cultivars 72 h post inoculation. Gene expression patterns in susceptible cultivars differed from those in potato varieties with a higher level of resistance. In general, a stronger gene induction was observed in the susceptible cultivars compared to the moderately to highly resistant potato varieties. Five genes with the highest homology to stress and/or defence-related genes were induced specifically in the susceptible cultivars. Four genes responded to pathogen attack independently of the level of resistance of the cultivar used, and three genes were repressed in infected tissue of most cultivars. Even in the absence of P. infestans infection, six genes showed higher expression levels in the somewhat resistant cultivars Bettina and Matilda. Possible reasons for the different levels of gene expression are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16388472</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1435-8603</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>6</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant biology (Stuttgart, Germany)</Title><ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation></Journal><ArticleTitle>Comparative analysis of Phytophthora infestans induced gene expression in potato cultivars with different levels of resistance.</ArticleTitle><Pagination><MedlinePgn>686-93</MedlinePgn></Pagination><Abstract><AbstractText>Differential gene expression was analyzed after infection with Phytophthora infestans in six potato cultivars with different levels of resistance to late blight. To verify the infection of the potato leaflets, the amount of phytopathogen mRNA within the plant material was quantified by real-time quantitative PCR. The expression of 182 genes selected from two subtracted cDNA libraries was studied with cDNA array hybridization using RNA from non-infected and infected potato leaflets. Gene up- and down-regulation were clearly detectable in all cultivars 72 h post inoculation. Gene expression patterns in susceptible cultivars differed from those in potato varieties with a higher level of resistance. In general, a stronger gene induction was observed in the susceptible cultivars compared to the moderately to highly resistant potato varieties. Five genes with the highest homology to stress and/or defence-related genes were induced specifically in the susceptible cultivars. Four genes responded to pathogen attack independently of the level of resistance of the cultivar used, and three genes were repressed in infected tissue of most cultivars. Even in the absence of P. infestans infection, six genes showed higher expression levels in the somewhat resistant cultivars Bettina and Matilda. Possible reasons for the different levels of gene expression are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ros</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, Center for Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 2, 85350 Freising, Germany. barbara.ros@wzw.tum.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thümmler</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Wenzel</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biol (Stuttg)</MedlineTA><NlmUniqueID>101148926</NlmUniqueID><ISSNLinking>1435-8603</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015854" MajorTopicYN="N">Up-Regulation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>1</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>4</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>1</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16388472</ArticleId><ArticleId IdType="doi">10.1055/s-2005-872946</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li></region><settlement><li>Munich</li></settlement><orgName><li>Université technique de Munich</li></orgName></list><tree><noCountry><name sortKey="Thummler, F" sort="Thummler, F" uniqKey="Thummler F" first="F" last="Thümmler">F. Thümmler</name><name sortKey="Wenzel, G" sort="Wenzel, G" uniqKey="Wenzel G" first="G" last="Wenzel">G. Wenzel</name></noCountry><country name="Allemagne"><region name="Bavière"><name sortKey="Ros, B" sort="Ros, B" uniqKey="Ros B" first="B" last="Ros">B. Ros</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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